Document Type : Original Research Paper


1 University of Tehran

2 Mine Faculty, Tehran university

3 geological survey

4 Tehran university



Microbial-leaching of nickel from two arsenide ores (low- and high-grade samples) was precisely investigated. The microbial leaching of nickeline was accomplished with the admixture of mesophilic cultures in shake flasks with pulp densities of 0.5, 1, and 5% as well as 0.5, 1, and 3% respectively. The nickel content was over 99% dissolved by mesophilic bacteria of low-grade and high-grade samples after 10 and 28 days respectively. In addition, three heterotrophic bacteria isolated from iron mine wastewater and tailing were used for bio-leaching of low and high- grade nickeline bearing arsenic ores. Three bacteria strains were investigated for their ability to leach from nickeline samples in different pulp densities (0.5%, 0.8%, 1.1%, and 3%)., Glutamicbacter nicotiana was more effective than the other strains. The Glutamicbacter nicotiana extracted Ni 100%, for the low-grade sample and up to 70 for the high-grade sample, respectively. The catalytic effect and galvanic interaction of different additives on the dissolution of the samples were investigated. In the low-grade sample, all four additives in both low-grade samples showed a positive efficiency. In high-grade, three of the four additives (silver-pyrite and L-Cysteine) had a positive effect on the dissolution of nickel.


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