The calpionellid biozonation within Shal and Kolur Formations in Shal region, SE Khalkhal are accomplished based on standard biozonation of Allemann et al. (1977). In this study, 5 biozones are introduced from Tithonian to Hauterivian:biozone. Biozone 1 belongs to Shal Formation and biozones 2 to 5 to Kolur Formation: 1- Crassicollaria intermedia Taxon Range Zone; Late Tithonian, 2- Calpionella alpina Acme Zone; Early Berriassian, 3- Calpionellopsis simplex - Calpionellites darderi Interval Zone; Late Berriassian to Early Valanginian, 4- Calpionellites darderi - Tintinopsella longa Interval Zone; Valanginian, 5- Tintinopsella longa - Tintinopsella carpathica Interval Zone; Late Valanginian- Hauterivian. Biozone 2 is also subdivided into the Calpionella alpina and Calpionella elliptica Subzones. Biozone 3 is subdivided into the Calpionellopsis simplex and Calpionellopsis oblonga Subzones. Based on this zonation, the Jurassic /Cretaceous boundary (Tithonian/ Berriassian) is defined at the Lower boundary of the Calpionella alpina zone which is equivalent to the Shal and Kolour Formations boundary.